High-throughput sorting of small objects via oil and/or moisture content using low-field nuclear magnetic resonance

ABSTRACT

The current disclosure describes an automated high-throughput seed sorting system for separating seed via oil and/or moisture content using novel nuclear magnetic resonance (NMR) systems and methods. The disclosed systems and methods for measuring the oil and/or moisture content of a single seed in a low-field time domain NMR instrument are superior in sample throughput and signal-to-noise ratio to conventional NMR systems and methods (free induction decay or spin echo) for single seed oil/moisture measurement.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No.14/206,238 filed on Mar. 12, 2014, which claims the benefit of U.S.Provisional Application No. 61/791,411, filed on Mar. 15, 2013, thedisclosure of which are incorporated herein by reference in theirentirety.

FIELD

The present disclosure relates generally to low-field time domain NMRsystems and methods for measuring oil and/or moisture content of smallobject samples (e.g., seed samples). More particularly, it relates toautomated systems and methods for using NMR relaxometry for thehigh-throughput continuous sorting of small objects (e.g., seeds) viameasuring the oil and/or moisture content dynamically.

BACKGROUND

The statements in this section merely provide background informationrelated to the present disclosure and may not constitute prior art.

Low-field NMR relaxometry has been increasingly used in many analyticalapplications, for example, determining oil and/or moisture content insmall objects, such as seeds, measuring xylene solubility inpolyethylene, and determining the solid to liquid fat ratio inmargarine. Conventional NMR methods for determining the oil/moisturecontent in small object samples using the Hahn spin-echo pulse techniquehas become an international standard method (AOCS, American Oil ChemistSociety, official method, 1995). Despite its wide use in analyticallaboratories for nondestructive oil measurement, the Hahn spin echo, andother known NMR based methods requires an undesirably long time for anindividual measurement.

SUMMARY

The current disclosure describes automated high-throughput small objectsorting systems and methods for separating small objects (e.g., seeds)via oil and/or moisture content using a nuclear magnetic resonance (NMR)method. The NMR systems and methods described herein are superior toconventional NMR methods (e.g., free induction decay or spin echo) fornondestructive single object oil and/or moisture measurement in terms ofsample throughput and signal-to-noise ratio. This nondestructiveanalytical technique has the potential of evaluating the oil and/ormoisture content in 30,000 to 50,000 individual small objects, or more,per hour using a low-field NMR relaxometer. The continuoushigh-throughput NMR-based systems and methods described herein providethe ability to rapidly and accurately measure the oil and/or moisturecontent in a moving small object.

For example, in various embodiments, the present disclosure provides anautomated high-throughput small object sorting system including a smallobject conveyor belt having a plurality of small object cups attachedthereto, wherein the conveyor assembly is structured and operable tocontinuously move the conveyor belt at a selected constant rate of speedduring operation of the system. The system additionally includes a smallobject feeder assembly that is structured and operable to singulatesmall objects from a plurality of small objects and deposit eachsingulated small object into a respective one of the small object cupsas the conveyor belt continuously moves at the selected constant rate ofspeed. The system further includes a nuclear magnetic resonance (NMR)assembly having the conveyor belt operably extending therethrough. TheNMR assembly is structured and operable to generate oil and/or moisturemass data for each small object as each small object moves through theNMR assembly at the selected constant rate of speed.

Still further, the system includes a microwave resonance cavity that isstructured and operable to receive and have pass therethrough, withoutpause, each small object after each respective small object has beenconveyed through the NMR assembly, and to obtain total small object massdata for each respective small object. Further yet, the system includesa computer based central control system that is structured and operableto: receive the at least one of oil and moisture mass data from the NMRassembly for each small object; receive the total small object mass fromthe microwave resonance cavity for each small object; and executeoil/moisture content software. Execution of the oil/moisture contentsoftware will store oil and/or moisture mass data for each small objectand associate the at least one of oil and moisture mass data receivedfor each small object with the respective small object. Execution of theoil/moisture content software will additionally store the total smallobject mass data for each small object and associate the total smallobject mass data for each small object with the respective small object.Furthermore, execution of the oil/moisture content software willcompute, based on the at least one of oil and moisture mass and totalmass data for each small object, an oil/moisture content value for eachrespective small object within a time period dictated by the selectedconstant rate of speed of the conveyor belt.

Although the systems and methods described herein can be used to measurethe oil and/or moisture content of various small objects, the presentdisclosure will exemplarily describe the inventive systems and methodswith regard to seeds. However, such exemplary embodiments anddescription should not be considered as limiting the scope of thepresent disclosure.

For example, continuous high-throughput NMR-based systems and methodsdescribed herein are particularly useful for separating haploid fromdiploid seeds generated with a high oil inducer, a key step in thedouble haploid breeding process. Separation of haploid from diploidseeds can be achieved based on oil content differences. A method ofmeasuring the oil content of a single seed in a low-field time domainNMR instrument is described in detail below.

Further areas of applicability of the present teachings will becomeapparent from the description provided herein. It should be understoodthat the description and specific examples are intended for purposes ofillustration only and are not intended to limit the scope of the presentteachings.

DRAWINGS

The drawings described herein are for illustration purposes only and arenot intended to limit the scope of the present teachings in any way.

FIG. 1 is an isometric view of a high throughput dynamic small objectsorting system that utilizes nuclear magnetic resonance to sort smallobjects based on oil and/or moisture content, in accordance with variousembodiments of the present disclosure.

FIG. 2 is an isometric view of a small object feeder assembly of thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 3A is an isometric view of the small object feeder assembly shownin FIG. 2 and a proximal end of a small object conveyor assembly of thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 3B is an isometric view of the proximal end of a small objectconveyor assembly shown in FIG. 3A, in accordance with variousembodiments of the present disclosure.

FIG. 4 is an isometric view of a distal end of the small object conveyorassembly of the high throughput dynamic small object sorting systemshown in FIG. 1, in accordance with various embodiments of the presentdisclosure.

FIG. 5 is a side view of a nuclear magnetic resonance assembly of thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 6A is an isometric view of a microwave resonance cavity and adiverter assembly of the high throughput dynamic small object sortingsystem shown in FIG. 1, in accordance with various embodiments of thepresent disclosure.

FIG. 6B is an isometric view of an inline microwave resonance cavity, inaccordance with various other embodiments of the present disclosure.

FIG. 7 is block diagram of a computer based central control system ofthe high throughput dynamic small object sorting system shown in FIG. 1,in accordance with various embodiments of the present disclosure.

FIG. 8 is an isometric view of one of a plurality of small object cupsof the high throughput dynamic small object sorting system shown in FIG.1, in accordance with various embodiments of the present disclosure.

FIG. 9A is a graph illustrating the periodic dependence of a NMRmagnetization signal on an offset O1, with two different Tp values, usedduring experiments utilizing the high throughput dynamic small objectsorting system shown in FIG. 1, in accordance with various embodimentsof the present disclosure.

FIG. 9B is a graphical illustration similar to that shown in FIG. 8performed on a single seed corn sample, in accordance with variousembodiments of the present disclosure.

FIG. 10 is a graphical illustration demonstrating a correlation betweenthe oil content (mass) and NMR signal amplitude generated duringexperiments utilizing the high throughput dynamic small object sortingsystem shown in FIG. 1, in accordance with various embodiments of thepresent disclosure.

FIG. 11A is a graphical illustration showing signal amplitude as afunction of total oil mass of the seed sample, during experimentsutilizing the high throughput dynamic small object sorting system shownin FIG. 1, in accordance with various embodiments of the presentdisclosure.

FIG. 11B is a graphical illustration showing signal amplitude per unitmass as a function of oil content (oil percentage) for seed samplesduring experiments utilizing the high throughput dynamic small objectsorting system shown in FIG. 1, in accordance with various embodimentsof the present disclosure.

FIG. 12 is a graphical illustration showing where an O1 frequency offsetwas plotted against the NMR signal, the NMR signal varies periodicallywith different O1 values, during experiments utilizing the highthroughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 13A is a graphical illustration showing reference oil weight (ingram) versus predicted oil weight (in gram) of a set of 36 calibrationsamples (real diploid/haploid seeds), during experiments utilizing thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 13B is a graphical illustration showing prediction residual foreach sample shown in FIG. 13A, in accordance with various embodiments ofthe present disclosure.

FIG. 14A is a graphical illustration showing reference oil weight (ingram) versus predicted oil weight (in gram) of a set of 78 validationsamples (real diploid/haploid seeds), during experiments utilizing thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 14B is a graphical illustration showing prediction residual foreach sample shown in FIG. 14A, in accordance with various embodiments ofthe present disclosure.

FIG. 15 is a graphical illustration showing the correlation between oilmass and NMR signal for seed traveling at various speeds, duringexperiments utilizing the high throughput dynamic small object sortingsystem shown in FIG. 1, in accordance with various embodiments of thepresent disclosure.

FIG. 16 is a graphical illustration showing that a validation study with78 doubled haploid seeds showed good correlation (R^2=0.982) between NMRsignal and oil mass, during experiments utilizing the high throughputdynamic small object sorting system shown in FIG. 1, in accordance withvarious embodiments of the present disclosure.

FIG. 17A is a graphical illustration of a ‘single-shot’ pulse sequenceimplemented by the high throughput dynamic small object sorting systemshown in FIG. 1, in accordance with various embodiments of the presentdisclosure.

FIG. 17B is a graphical illustration of signal generated for a corn seedutilizing the ‘single-shot’ pulse sequence implemented by the highthroughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 18 is a graphical illustration showing a comparison of calibrationcurves implementing the by the high throughput dynamic small objectsorting system shown in FIG. 1, using as single-shot analysis method andSSFP analysis method, in accordance with various embodiments of thepresent disclosure.

FIG. 19 is a graphical illustration showing a comparison of magneticfield dependency implementing the by the high throughput dynamic smallobject sorting system shown in FIG. 1, using as single-shot analysismethod and SSFP analysis method, in accordance with various embodimentsof the present disclosure.

FIG. 20 is a graphical illustration showing a correlation between asingle-shot analysis NMR signal and oil mass implementing the by thehigh throughput dynamic small object sorting system shown in FIG. 1, inaccordance with various embodiments of the present disclosure.

FIG. 21 is a graphical illustration showing the data of FIG. 20 whensamples with extremely low oil content are removed, in accordance withvarious embodiments of the present disclosure.

FIG. 22 is a graphical illustration of a typical bimodal distribution ofoil percentage with Gaussian least-squares curve fitting resultsoverlaid with the peaks labeled as “P1” and “P2”, in accordance withvarious embodiments of the present disclosure.

FIG. 23 is a graphical illustration of haploid recovery and haploidpurity vs. oil percentage sorting threshold estimated from numericalintegration of peaks “P1” and “P2”, in accordance with variousembodiments of the present disclosure.

FIG. 24 is a graphical illustration showing a correlation for 60distinct populations between haploid recovery measured by field growoutdata and predicted haploid recovery from Gaussian curve fitting of apopulation-specific training set, in accordance with various embodimentsof the present disclosure.

FIG. 25 is a graphical illustration showing a correlation for 60distinct populations between haploid purity measured by field growoutdata and predicted haploid purity from Gaussian curve fitting of apopulation-specific training set, in accordance with various embodimentsof the present disclosure.

Corresponding reference numerals indicate corresponding parts throughoutthe several views of drawings.

DETAILED DESCRIPTION

The following description is merely exemplary in nature and is in no wayintended to limit the present teachings, application, or uses.Throughout this specification, like reference numerals will be used torefer to like elements.

Described herein are systems and methods for the ultra-fastdetermination of the oil and/or moisture content of a single smallobject (e.g., a single seed). Generally, the systems and methodsdescribed herein employ a train of 90° pulse sequences, or alternativelya combination of a single 90° pulse and a train of 180° pulses, toproduce an NMR signal in a low-field NMR relaxometer. The amplitude ofthe NMR signal obtained is directly proportional to the oil and/ormoisture content of the object. Due to the significant improvement ofsignal-to-noise ratio (S/N), the disclosed NMR systems and methods canaccurately determine the oil and/or moisture content of a single smallobject sample (e.g., a single seed sample) within an extremely shorttime period, e.g., within 5 to 30 milliseconds, which enables thehigh-throughput sorting of objects based on their oil and/or moisturecontent difference. Additionally, the disclosed systems and methodsallow object samples to be measured in a continuous and dynamic fashion,thereby making automatic high-speed sorting by NMR possible.

Referring now to FIG. 1, the present disclosure provides a highthroughput dynamic small object sorting system 10 that utilizes nuclearmagnetic resonance to sort small objects based on oil and/or moisturecontent. Generally, the system 10 includes a feeder assembly 14, aconveyor assembly 18, a nuclear magnetic resonance (NMR) assembly 22, amicrowave resonance cavity 26, a diverter assembly 30, and a computerbased central control system 34. The control system 34 is structured andoperable to directly or indirectly control and coordinate the automatedand cooperative functions and operations of the feeder assembly 14, theconveyer assembly 18, NMR assembly 22, the microwave resonance cavity26, and the diverter assembly 30, as described below. The control system34 is further structured and operable to execute one or more oil and/ormoisture content analysis programs or algorithms (simply referred toherein as oil/moisture content analysis software) for analyzing datagenerated and collected by the system 10 to identify and separate smallobjects (e.g., separate haploid seeds from diploid seeds) based on oiland/or moisture content, at a high rate of speed, e.g., 5 to 30milliseconds/seed, as also described further below.

More specifically, the system 10, as controlled by the central controlsystem 34, is structured and operable to singulate small objects (e.g.,seeds) from a plurality of small objects (e.g., seeds) via the feederassembly 14, dynamically transport the singulated small objects throughthe NMR assembly 22 at a high rate of speed via the conveyor assembly18, collect oil and/or moisture mass data for each individual object asthe objects are dynamically transported through the NMR assembly 22,collect total object mass data for each individual object via themicrowave resonance cavity 26, calculate the oil and/or moisture contentfor each individual small object, and separate the small objects (e.g.,separate haploid seeds from diploid seeds) based on the respective oiland/or moisture content via the diverter assembly 30, at a high rate ofspeed, e.g., 20 to 50 seeds per second.

It should be understood that although the system 10, as describedherein, can be used to measure the oil and/or moisture content ofvarious small objects, for simplicity and clarity the present disclosurewill exemplarily describe the system 10 for use in measuring the oilcontent of seeds. However, such exemplary embodiments and descriptionshould not be considered as limiting the scope of the presentdisclosure.

Additionally, it is envisioned that the system 10 described herein caninclude additional and/or alternative sensing and detection technologiesto measure characteristics of the small objects (e.g., seeds) other thanmoisture and/or oil content, such that the small objects (e.g., seeds)can be sorted based on moisture content and/or oil content and/or someother identifiable and distinguishable characteristic(s) of the smallobjects (e.g., seeds), and remain within the scope of the presentdisclosure.

Referring now to FIGS. 1, 2, 3A and 3B, in various embodiments, thefeeder assembly 14 (hereafter the seed feeder assembly 14) comprises abulk seed hopper 38, a seed singulator 42, and a rotating seeddispensing sprocket 46. The bulk seed hopper 38 is structured andoperable to retain a desired quantity of selected seeds and to feed theseeds into the singulator 42. The singulator 42 is structured andoperable to singulate individual seeds 48 from the hopper 38, i.e.,parse, or separate, the seeds 48 one-by-one from the plurality of seedsin the hopper 38. The singulator 42 is further structured and operableto place each singulated seed 48 into a respective one of a plurality ofseed reservoirs 50 formed in the periphery of the dispensing sprocket46. More particularly, during operation of the seed feeder assembly 14,the dispensing sprocket 46 is rotated about a dispensing sprocket axle54. As the dispensing sprocket 46 rotates, the singulator 42 depositseach singulated seed 48, i.e., parsed seed 48, into a respective one ofthe seed reservoirs 50. Subsequently, as the dispensing sprocket 46continues to rotate, each seed 48 that has been deposited into one theseed reservoirs 50 is deposited into a respective one of a plurality ofseed cups 58 that are connected to a conveyor belt 62 of the seedconveyor assembly 18, as described further below.

Referring now to FIGS. 1, 2, 3A, 3B, 4 and 8, in various embodiments theseed conveyor assembly 18 includes the seed cups 58 connected to theconveyor belt 62 that is slidingly disposed within a conveyor track 66,and at least one drive wheel 70 operably connected to at least one drivemotor (not shown) to drive the conveyor belt 62 along the track 66,i.e., cause the conveyor belt 62 and seed cups 58 to travel along thetrack 66. More specifically, the drive motor(s) are structured andoperable, as controlled by the central control system 34, to drive theconveyor belt 62 at a constant rate of speed, e.g., 0.5 to 2 meters persecond, such that the seed cups 58, and more importantly the singulatedseeds 48 disposed in the seed cups 58, are conveyed, or transported,from a proximal end 66A of the conveyor track 66, through the NMRassembly 22, to a distal end 66B of the convey track at a constant rateof speed. For example, in various embodiments, the drive motor(s) arecontrolled by the central control system 34 to drive the conveyor belt62 at a selected rate of speed such that each seed 48 is deposited intothe respective seed cup 58 at the conveyor belt track proximal end 66Aand conveyed through the NMR assembly 22 to the conveyor belt trackdistal end 66B of the conveyor track 66 within approximately 1 second,or alternatively approximately 0.5 to 1.5 seconds. In variousimplementations, the conveyor assembly 18 additionally includes at leastone passive belt guide wheel 74 to guide the conveyor belt 62 as ittravels beneath the conveyor belt track 66 and a track cover 78 thatcovers the conveyor belt track 66 and prevents seeds 48 from beingdislocated from the respective seed cups 58.

Referring now to FIG. 5, in various embodiments, the NMR assembly 22comprises a housing 82 that encloses a nuclear magnetic resonance (NMR)relaxometer 86, i.e., a nuclear magnet, and a radio frequency (RF) probe90, i.e. a RF transceiver. In various embodiments, the NMR relaxometer86 has a longitudinal length L of approximately 0.50 to 1.5 meters,e.g., approximately 0.78 meters, and the relaxometer plus the RF probehave an overall length of between 0.75 and 2.0 meters, e.g., 1.1 meters.As described above, the conveyor belt 62 conveys the seeds 48 throughthe NMR assembly 22 at a selected constant rate of speed, e.g., 1.0-1.5meters/second. More particularly, the conveyor belt 62, as controlled bythe central control system 34, conveys the seeds 48 past, or through,the entire length of NMR relaxometer 86 and past, or through, the RFprobe 90 at the selected constant rate of speed. During operation of thesystem 10, as the seeds 48 are conveyed past/through the NMR relaxometer86, at the selected constant rate of speed, a magnetic field generatedby the NMR relaxometer 85 is exerted on the seeds 48. The magnetic fieldcauses the protons of each respective seed 48 to align parallel to thedirection of the magnetic field.

Importantly, and as described further below in Experiment No. 2, theseeds 48 are moving at a constant rate of speed via the conveyor belt 62and only pass through the NMR assembly 22 once. That is, the seeds 48are never static as is the case with a traditional NMR pulse sequencethat scans a static sample multiple times. Moreover, given the extendedlength L of the NMR relaxometer 86, e.g., 0.5-1.5 meters, and theconstant rate of speed of conveyance of each seed 48 through the NMRrelaxometer, e.g., 1.0-1.5 meters/second, each seed 48 is exposed to theNMR magnetic field, i.e., each seed 48 is polarized, for an extendedperiod of time, e.g., 0.5-3.0 seconds. Accordingly, each constantlymoving seed 48 is exposed to the NMR magnetic field for a sufficienttime for the protons of each respective seed 48 to align parallel to thedirection of the magnetic field without slowing or stopping the rate ofspeed of conveyance of each seed 48.

Subsequently, as the seeds 48 continue to move through and along thelength of the NMR relaxometer 86 and pass through the RF probe 90 at theselected constant rate of speed, the RF probe 90 generates one or morepulses and receives an echo from each pulse. For example, the RF probe90 can generate a plurality of pulses at any desired interval, e.g., 5pulses/millisecond, or approximately 100-200 microseconds betweenpulses, and receive an echo from each pulse. Each pulse disrupts, ordisturbs, the proton alignment, whereby the amount of proton disruptionis identified in the echo received from each pulse. The central controlsystem 34, via execution of the oil content analysis software, utilizesthe amount of proton disruption from each echo to generate dataindicative of the mass of oil in each respective seed, hereafterreferred to oil mass data.

Note, that as described above, it is envisioned that the system 10 canbe used to measure oil content and/or moisture content (and/or otherdistinguishable characteristics) in various small objects other thanseeds. Hence, in such alternate embodiments, the central control system34 would execute oil and/or moisture content analysis software, toutilize the amount of proton disruption from each echo to generate dataindicative of the mass of oil and/or moisture in each respective smallobject.

Continuing now with the exemplary seed embodiment, subsequently, viaexecution of the oil content analysis software, the central controlsystem 34 records and saves the oil mass data, i.e., the oil mass datafrom each pulse echo and associates, links or ties the oil mass data tothe respective seed 48. Hence, seeds 48 are constantly moving at theselected constant rate of speed, e.g., 1 m/sec, (that is, in a dynamicstate as opposed to a static state) as the protons are aligned, via theNMR relaxometer 86, and the oil mass data is generated, via the RF probe90, and the oil mass data is gathered and saved, via the central controlsystem 34.

It should be noted that by generating the RF pulses and receiving theechoed oil mass data at high rate, e.g., 5 pulses/millisecond, noise insubsequent detected echoes partially cancel each other out, therebysignificantly increasing the signal-to-noise ratio of the NMR signalmeasurement, allowing more accurate and robust measurement of oilcontent in the respective seeds, as described herein.

Referring now to FIG. 6A, as described above, the system 10 includes themicrowave resonance cavity 26 and the diverter assembly 30. The system10 additionally includes an offload funnel 94 mounted to the distal end66B of the conveyor track 66, and/or other system 10 structure locatedat or near the conveyor track distal end 66B. The offload funnel 94includes an ingress end 94A and an egress end 94B. The offload funnel 94is structured and operable to receive seeds 48 that are offloaded fromthe respective seed cups 58 as the conveyor belt 62 and seed cups 58travel along the periphery of the drive wheel 70 at the distal end 66Bof the convey track 66. More specifically, after each seed 48 has beenconveyed through the NMR assembly 22 and the respective oil mass datahas been gathered, saved and associated with the respective seed 48, asdescribed above, each seed cup 58 and respective seed 48 is conveyedalong the conveyor track distal end 66B and along the periphery of thedistal end drive wheel 70. As each seed cup 58 travels along theperiphery of the distal end drive wheel 70, the respective seed 48 fallsout of the seed cup 58 into the offload funnel ingress end 94A.

In various embodiments, the diverter assembly 30 is connected to themicrowave resonance cavity 26, wherein the egress end 94B of the offloadfunnel 94 is connected to the microwave resonance cavity 26.Accordingly, once each seed falls out of the respective seed cup 58 andinto the offload funnel ingress end 94A, due to the force of gravity,the seeds 48 fall through the offload funnel 94 and are directed intothe microwave resonance cavity 26 via the offload funnel egress end 94B.Alternatively, air can be blown through the offload funnel 94 in thedirection of seed travel to assist and/or accelerate the travel speed ofthe seeds 48 through the offload funnel 94. In such embodiments, afterthe seeds 48 exit the offload funnel 94 and enter the microwaveresonance cavity 26, each seed 48 falls through via gravity, oralternatively is accelerated through via forced air, an internal passage(not shown) of the microwave resonance cavity 26 and enters the diverterassembly 30.

The microwave resonance cavity 26 is structured and operable toaccurately measure, or predict, the total mass of each seed 48 as itpasses through the internal passage. The microwave resonance cavity 26communicates the total mass data of each seed 48 to the central controlsystem 34, where, via execution of the oil content analysis software,the central control system 34 records and saves the total mass data fromeach seed 48 and links or ties the total mass data to the respectiveseed 48 and the previously saved oil mass data for the respective seed48.

Importantly, via execution of the oil content analysis software, thecentral control system 34 utilizes the saved oil mass data and totalmass data to compute the oil content of each seed 48 as each respectiveseed 48 passes through the microwave resonance cavity 26. Hence, priorto each seed 48 exiting the microwave resonance cavity 26, the centralcontrol system 34 computes the oil content of the respective seed 48.Moreover, prior to each seed 48 exiting the microwave resonance cavity26, based on the computed oil content of the respective seed 48, thecentral control system 34 determines whether the oil content ofrespective seed 48 exceeds an oil content threshold for the respectiveseed. That is, based on the computed oil content of the respective seed48, the central control system 34 determines whether the oil content ofrespective seed 48 indicates that the seed is haploid or diploid.

Upon exiting the microwave resonance cavity 26, each seed 48 enters thediverter assembly 30 where, as controlled by the central control system34, the diverter assembly 30 directs or diverts seeds 48 with an oilcontent below the respective threshold, i.e., haploid seeds, to ahaploid receptacle 98 and directs or diverts seeds 48 with an oilcontent above the respective threshold, i.e., diploid seeds, to adiploid receptacle 102. Generally, the diverter assembly 30 includes ahollow central tube 106 that is connected at a proximal end to themicrowave resonance cavity 26 (in fluid connection with the microwaveresonance cavity internal passage), a diverter device 110 cooperativelyconnected to the central tube 106, a diverted seed funnel 114 connectedto the central tube 106 (in fluid connection with an interior lumen ofthe central tube 26), and a non-diverted seed funnel 118 disposed at adistal end of the central tube 106 (in fluid connection with theinterior lumen of the central tube 26). In operation, as each seed 48exits the microwave resonance cavity 26 each seed 48 enters the interiorlumen of the hollow central tube 106, whereafter, based on the computedoil content of each respective seed 48, each respective seed 48 iseither diverted or directed into the diverted seed funnel 114, viaoperation of the diverter device 110 (as controlled by the centralcontrol system 34), or is allowed to fall through the central tube intothe non-diverted seed funnel 118.

For example, in various embodiments, seeds 48 that are determined to bediploids, based on the computed oil content of the respective seeds 48,are diverted into the diverted seed funnel 114, whereafter the diploidseeds 48 pass through the diverted seed funnel 114 and are depositedinto the diploid receptacle 102. Conversely, seeds 48 that aredetermined to be haploids, based on the computed oil content of therespective seeds 48, are allowed to fall through the central tube 106into the non-diverted seed funnel 118, whereby the haploid seeds 48 aredeposited into the haploid receptacle 98. In alternative embodiments,execution of the oil content analysis software could operate thediverter device 110 to divert or direct haploid seeds 48 into thediverted seed funnel 114 and allow diploid seeds 48 to fall through tothe non-diverted seed funnel 118.

The diverter device 110 can be any device suitable for diverting ordirecting selected seeds 48 (e.g., diploid seeds) into the diverted seedfunnel 114 and allow other selected seeds 48 (e.g., haploid seeds) tofall through the central tube 106 into the non-diverted seed funnel 118.For example, in various embodiments, the diverter device 110 can be apneumatic device that controls a flow of air to divert the selectedseeds 48 into the diverted seed funnel 114. Particularly, in suchembodiments, if a seed 48 is determined to be a diploid while therespective seed 48 passes through the microwave resonance cavity 26, asthe respective diploid seed 48 falls through the diverter device centraltube 106, a valve of the pneumatic diverter device opens such that airis released that diverts or directs the respective diploid seed 48 intothe diverted seed funnel 114, whereafter the respective diploid seed 48is expelled into the diploid receptacle 102. While conversely, if a seed48 is determined to be a haploid while the respective seed 48 passesthrough the microwave resonance cavity 26, as the respective diploidseed 48 falls through the diverter device central tube 106, the valve ofthe pneumatic diverter device remains closed such that no air isreleased and the respective haploid seed 48 falls through the divertedseed funnel 114 into the haploid receptacle 98. Other electro-mechanicaltype diverter devices 110 are envisioned and within the scope of thepresent disclosure.

In various embodiments, it is envisioned that the oil/moisture contentanalysis software and the diverter assembly 30 can be structured andoperable to divert seeds (or small objects) to more than two differentreceptacles.

Referring now to FIG. 6B, in various embodiments, the microwaveresonance cavity 26 can be an inline microwave resonance cavity 26′disposed near the distal end 66B of the conveyor belt 66. In variousimplementations, the inline microwave resonance cavity 26′ can bestructured to such that the conveyor belt 66, and hence the seed cups 58and respective seeds 48 deposited in each seed cup 58 pass through aninternal passage 146 of the inline microwave resonance cavity 26′.Generally, as described above with regard to the microwave resonancecavity 26, the inline microwave resonance cavity 26′ is structured andoperable to accurately measure, or predict, the total mass of each seed48 as it passes through the internal passage 146. The microwaveresonance cavity 26 communicates the total mass data of each seed 48 tothe central control system 34, where, via execution of the oil contentanalysis software, the central control system 34 records and saves thetotal mass data from each seed 48 and links or ties the total mass datato the respective seed 48 and the previously saved oil mass data for therespective seed 48.

More specifically, in such embodiments, the conveyor belt 62 passesthrough the internal passage 146 of the inline microwave resonancecavity 26′ and the combined mass of conveyor belt 62, seed cup 58, andseed 48 is measured for each seed position. The belt plus the seed cuponly mass for each seed position is tabulated prior to seed loading foreach run and the seed 48 mass is inferred from the difference of themass measured by the inline microwave resonance cavity 26′ and belt/seedcup-only mass. In such embodiments the diverter assembly 30 is connectedto the egress end 94B of the offload funnel 94 and is structured andoperable as described above.

Referring to FIG. 8, in various embodiments, each seed cup 58 isstructured and operable to help settle, center and retain eachsingulated seed 48 in a stable orientation within the respective seedcup 58 as and after each seed 48 is deposited into the respective seedcup 58, as described above. For example, in various embodiments, eachseed cup 58 is formed to comprise a 3-dimensional (3D) diamond shapedreservoir 122 into which each respective seed 48 is deposited. Thebeveled and angled sides of the 3D diamond reservoir 122 cause eachsingulated seed 48 to be centered within the respective seed cup 58.Although the reservoir 122 is shown and described to be diamond shaped,it is envisioned that the reservoir 122 can be any other shape suitableto retain the seeds 48 within the seed cups 58 in a stable orientationduring conveyance of the seeds 48 along the seed track 66 and throughthe NMR assembly 22. Additionally, in various embodiments, each seed cup58 comprises a plurality of lateral slots, grooves or serrations 126extending through the body of the respective seed cup 58, wherein theserrations 126 create sufficient friction between each respective seed48 and the beveled and angled sides of the 3D diamond shaped (or othersuitably shaped) reservoir 122 to reduce vibration of the respectiveseed 48 as the seed 48 and seed cup 58 travel along the conveyor track66. More particularly, the 3D diamond shaped (or other suitably shaped)reservoir 122 and serrations 126 are structured and operable to stablyretain the seeds 48 and reduce vibration of the seeds 48 such that theseeds 48 are prevented from ‘bouncing’ out of the seed cups 58 and areretained within the seed cups 58 in a stable orientation duringconveyance of the seeds 48 along the seed track 66 and through the NMRassembly 22.

Referring now to FIGS. 3A and 3B, in various embodiments, the system 10includes a stray seed removal assembly 130 that is structured andoperable, as controlled by the central control system 34, to identifystray seeds 48 that, upon dispensing from the seed feeder assembly 14,have missed or fallen out of the respective seed cup 58, andconsequently settle on the conveyor belt 62 between adjacent seed cups58. In various embodiments, the stray seed removal assembly 130 includesa stray seed sensor 134 that is structured and operable to detect strayseeds 48 that have settled onto the conveyor belt 62 between adjacentseed cups 58. The stray seed sensor 134 communicates such detection ofstray seeds 48 to the central control system 34. The stray seed removalassembly 130 additionally includes a stray seed expulsion device 138disposed on a first side of the conveyor track 66 and a stray seed catchfunnel 142 disposed opposite the stray seed expulsion device 138 on anopposing side of the conveyor track 66. The stray seed expulsion device138 can be any device suitable to remove or expel each stray seed 48from the conveyor belt 62 and deposit the removed or expelled strayseeds 48 into the stray seed catch funnel 142, whereby the removed orexpelled stray seeds 48 are directed into a stray seed receptacle (notshown).

For example, in various implementations, the stray seed expulsion device138 can be a pneumatic device that controls a lateral flow of air acrossthe conveyer belt 62 to expel the stray seeds 48 from the conveyor belt62 into the stray seed funnel 142. Particularly, in such embodiments, ifa stray seed 48 is detected by the stray seed sensor 134, as therespective stray seed 48 passes in between the stray seed expulsiondevice 138 and the stray seed catch funnel 142, a valve of the pneumaticstray seed expulsion device 138 (as controlled by central control system34) opens such that air is released that blows or expels the respectivestray seed 48 off the conveyor belt 62 into the stray seed catch funnel142, whereafter the respective stray seed 48 is directed into the strayseed receptacle.

Referring now to FIG. 7, in various embodiments, the central controlsystem 34 is a computer based system that generally includes at leastone processor 150 suitable to execute all software, programs,algorithms, etc., described herein to automatically, or robotically,control the operation of the high throughput dynamic seed sorting system10, as described herein. The central control system 34 additionallyincludes at least one electronic storage device 154 that comprises acomputer readable medium, such as a hard drive or any other electronicdata storage device for storing such things as software packages orprograms and algorithms 156 (e.g., the oil content analysis software),and for storing such things as digital information, data, look-uptables, spreadsheets and databases 158. Furthermore, the central controlsystem 34 includes a display 160 for displaying such things asinformation, data and/or graphical representations, and at least oneuser interface device 162, such as a keyboard, mouse, stylus, and/or aninteractive touch-screen on the display 158. In various embodiments thecentral control system 34 can further include a removable media reader166 for reading information and data from and/or writing information anddata to removable electronic storage media such as floppy disks, compactdisks, DVD disks, zip disks, flash drives or any other computer readableremovable and portable electronic storage media. In various embodimentsthe removable media reader 166 can be an I/O port of the central controlsystem 34 utilized to read external or peripheral memory devices such asflash drives or external hard drives.

In various embodiments, the central control system 34, i.e., theprocessor 150 can be communicatively connectable to a remote servernetwork 170, e.g., a local area network (LAN), via a wired or wirelesslink. Accordingly, the central control system 34 can communicate withthe remote server network 170 to upload and/or download data,information, algorithms, software programs, and/or receive operationalcommands. Additionally, in various embodiments, the central controlsystem 34 can be structured and operable to access the Internet toupload and/or download data, information, algorithms, software programs,etc., to and from internet sites and network servers.

In various embodiments, as described above, the central control system34 includes the oil content analysis software, which is stored on thestorage device 154 and executed by processor 150, and can furtherinclude one or more system control algorithms, or programs, stored onthe storage device 154 and executed by processor 150. The oil contentanalysis software and other system control software are cumulativelyidentified in FIG. 7 by the reference numeral 156. It should beunderstood that although the central control system 34 has beensometimes described herein as directly controlling the variousautomated, or robotic, operations of the high throughput dynamic seedsorting system 10, it is execution of the oil content analysis softwareand other system control software, programs and/or algorithms by theprocessor 150, using inputs from the user interface 162 and variousother components, sensors, systems and assemblies of the high throughputdynamic seed sorting system 10 that actually control the variousautomated, or robotic, operations of the high throughput dynamic seedsorting system 10 described herein.

Experimental Results

Experiments were performed utilizing the high throughput dynamic seedsorting system 10 to test the speed and accuracy of the high throughputdynamic seed sorting system 10 and methods for separating haploid seedsfrom diploid seeds. The experiments and results are described below

Experiment No. 1

Experimental results using a first analysis method, e.g., a steady statefree procession (SSFP) method, are as follows. For the followingexperiment, the RF probe 90 of NMR assembly 22 was configured as an 18mm probe (Dead1=15 μs, Dead2=15 μs, and 90° pulse duration P90=4.9 μs)operating at 40° C., and the magnetic field strength generated by theNMR relaxometer 86 was set to 0.55 Tesla, corresponding to a 1H Larmorfrequency of 23.4 MHz.

To test and optimize the data acquisition parameters, an artificial oilsample containing 0.3 g of tissue paper and 0.3 g of corn oil was used.To develop an oil calibration model for single seed corn samples, a setof 24 corn seeds with known oil content were used.

To obtain NMR signals with best signal-to-noise ratio, two keyparameters need to be optimized. These parameters were the RFtransmitter offset (O1) and the time interval between pulses (Tp). Tofind the optimal values of O1 and Tp, a series of experiments wereperformed with a set of different O1 and Tp values on the artificial oilsample. FIG. 9A shows the periodic dependence of NMR magnetizationsignal on the offset O1, with two different Tp values (100 μs and 300μs). Similar experiments were also performed on a single corn seedsample and the results are shown in FIG. 9B. Based on these results, theoffset O1 and time interval Tp were set to 3.37 and 100 μs,respectively, for subsequent quantitative oil calibration experiments.

To characterize the quantitative performance of the methods describedherein for determination of oil content, a set of 11 artificial oilsamples with oil mass varied from 5 mg to 40 mg were prepared, theamount of oil in each sample was measured using an analytical balance.The NMR methods described above were used to obtain the NMR signals ofthese samples. FIG. 10 demonstrates an excellent correlation between theoil content (mass) and the NMR signal amplitude. Particularly, FIG. 10illustrates NMR signal versus oil mass of the artificial oil samples.Each sample was measured in triplicate and the standard deviations wereused for the error bars. The small error bars in FIG. 10 indicates thatthe NMR signals are very reproducible.

It is important to develop a quantitative calibration model fordetermination of oil content in a single corn seed sample. A set of 24single corn seed samples was prepared. The oil contents of these sampleswere previously determined using a conventional NMR method (FID-Hahnmethod) and the measured values were used as reference values to assessthe performance of the experiments. The NMR signals were acquired withkey NMR parameters: Tp=100 μs, O1=3.3π. FIG. 11A shows the signalamplitude as a function of total oil mass of the seed sample. FIG. 11Bshows the signal amplitude per unit mass as a function of oil content(oil percentage) for seed sample. Each sample was measured in triplicateand the standard deviations were plotted as error bars in FIGS. 11A and11B. The small error bar in FIGS. 11A and 11B indicate that the NMRsignals utilizing the high throughput dynamic seed sorting system 10have good reproducibility.

Since the primary application of the high throughput dynamic seedsorting system 10 and methods described herein are for diploid/haploidseed sorting by measuring the oil content of single seed, the system 10and methods need to be optimized for the type of seed will beencountered in real sorting application. The method optimizationincludes pulse interval (Tp) adjustment and receiver frequency offset(O1) optimization. For Tp adjustment, Tp was adjusted to 150 μs to allowthe center of NMR signal at each pulse interval to be acquired. For O1optimization, the NMR measurement is done on a single seed. A sequenceof O1 values was applied and the corresponding NMR signals weremeasured. As shown in FIG. 12, where the O1 frequency offset was plottedagainst the NMR signal, the NMR signal varies periodically withdifferent O1 values. The optimal O1 value was set to 3800 Hz (or offsetangle π) because at this O1 value, the largest NMR signal amplitude wasobtained.

Because the oil range of real diploid/haploid seeds is smaller than thatof the previous calibration sample set (24 seeds), to ensure bestmeasurement accuracy, a new NMR calibration was developed using the realdiploid/haploid seeds. A set of 36 diploid/haploid seeds were used todevelop a new calibration model. For each seed, the oil content (inpercentage) was measured by a traditional NMR method and the mass wasdetermined by an analytical balance. The reference total oil contents(oil mass) were calculated by multiplying the oil percentage with themass of seed. The experiment was performed utilizing the system 10 toacquire NMR signal of each seed. For each seed, the measurements wererepeated 3 times to assess the measurement reproducibility (analyticalprecision).

To further improve the measurement reproducibility, the single seed wasconstrained at the center of the NMR assembly 22 by placing the seed onthe top of a sample holder in the NMR assembly 22, while the orientationof the seed was changed at each measurement. A univariate calibrationmodel was developed by correlating the reference total oil content withNMR signal (mean value of the 3 repeat measurements). The plot ofreference oil vs. prediction oil and the plot of prediction residualsare shown in FIGS. 13A and 13B. The calibration model shows that thereexists a very good correlation between reference oil mass and predictedoil mass (R2=0.977, standard error of calibration SEC=0.64 mg) and thatthe measurement reproducibility is very good (mean standarddeviation=0.34 mg).

To validate the NMR calibration, a set of 78 diploid/haploid seeds wereused. The reference oil weights of these seeds were determined asdescribed previously. As shown in FIGS. 14A and 14B, using the newcalibration model, the oil content can be predicted with great accuracy(R2=0.97, standard error of prediction SEP=0.69 mg) and good precision(mean standard deviation=0.3 mg).

In one embodiment, a dual-magnet NMR system that consists of twoidentical 0.5 Tesla magnets, one used as prepolarization magnet andanother used as measurement magnet, was constructed. An automatedhigh-speed linear convey system was connected to the NMR system todeliver corn seeds at speed up to 50 cm/s. A position trigger wasmounted on the conveyor track to trigger the NMR pulse at precise sampleposition. A set of 24 seeds were used to test the performance of thehigh-speed NMR prototype with sample moving at various speeds. FIG. 15shows the correlation between oil mass and NMR signal for seed travelingat various speeds. A set of 78 double haploid seeds were used tovalidate the performance of the system. These samples were moving at aspeed of 50 cm/s. FIG. 16 illustrates that the validation study with 78doubled haploid seeds showed good correlation (R^2=0.982) between NMRsignal and oil mass.

In conclusion, the experiments described above demonstrate that the highthroughput dynamic seed sorting system 10 and methods described hereinprovide a very useful analytical system and method for rapid andquantitative determination of oil content in single seed samples. Underthe optimal parameters, a linear relationship between the NMR signal andoil content can be found for seed samples. It was also shown that asuccessful calibration model was developed for determining the oilcontent in real diploid/haploid corn seeds. The NMR method was validatedon a large set of samples (78 diploid/haploid seeds) under high-speedand continuous measurement condition, and the result demonstrated thatthe high throughput dynamic seed sorting system 10 is able to determinethe oil content in single seed with great accuracy and precision.Additionally, as described above, due to the improvement ofsignal-to-noise ratio this NMR method, the measurement time for eachsingle seed sample can be as short as 20 ms. Thus, the high throughputdynamic seed sorting system 10 and methods described herein allow forsingle seed samples to be analyzed in an automated and continuousfashion.

Experiment No. 2

Experimental results using a second analysis method are as follows. Inorder to reach an analytical throughput of more than 20 seeds persecond, seed samples can only pass through the NMR assembly 22 once witha travel speed of more than 100 cm per second. Because each sampletravels through the NMR assembly 22 only once, there is no possibilityof using a traditional NMR pulse sequence that scans a static samplemultiple times. The methods of the present high-throughput measurementmust be ‘single-shot’. Therefore, in various embodiments, the highthroughput dynamic seed sorting system 10 described above can implementa single-shot method that generates a pulse sequence that comprises a 90degree pulse followed by a train of 180 degree pulses, as shown in FIG.17A. As shown in FIG. 17B, for a seed sample, the single-shot signalcontributed by moisture (water) component decays completely after 2 ms,the later part of single-shot signal is mainly contributed by oilcomponent. By averaging the later part of single-shot signal, a linearrelationship can be established between the averaged NMR signal and oilcontent.

Referring now to FIG. 18, to test the single-shot analysis method, a setof 24 seed samples with oil content varying from 3 mg to 50 mg were usedas a calibration set to evaluate the correlation between the single-shotsignals and oil content. The single-shot NMR measurement was utilizingthe high throughput dynamic seed sorting system 10, with the samplestraveling through the NMR assembly 22 at a speed of 50 cm per second.Each sample was measure 3 times and the standard deviation of NMR signalwas plotted as error bar in the calibration curve. The calibration curveusing single-shot method was compared with that using SSPF method, asshown in FIG. 18.

In addition to the superior signal to noise ratio (S/N) and betteranalytical performance, the single-shot method is more robust than theSSFP method. The single-shot signal is less dependent on magnetic fielduniformity in comparison to the SSFP method. FIG. 19 shows thedependency of the single-shot and the SSFP signals on the fieldfluctuation. Because the single-shot method has better tolerance ofmagnetic field fluctuation, it makes the magnetic field stabilityproblem less important and allows less expensive magnet to be used inthe NMR assembly 22.

To further validate the performance of single-shot method forquantitative determination of oil content, a set of 480 corn seeds,including both diploid and haploid seeds, were tested using the highthroughput dynamic seed sorting system 10 with the samples travelingthrough the NMR assembly 22 at a speed of 50 cm per second. FIG. 20shows the correlation between single-shot NMR signal and oil content.FIG. 21 shows the percentage based calibration curve using single-shotmethod.

Hence, as described above, the single-shot method was tested andvalidated for nondestructive and high-throughput determination of oilcontent in single seed. And, based on the results, as described above,the single-shot method is an excellent analytical method in terms ofaccuracy and precision. In addition, compared to the SSFP method, thesingle-shot method is more sensitive and robust.

Additionally, although the high throughput dynamic seed sorting system10 has been described above to use oil mass and seed mass to compute theoil content as marker to obtain superior separation between haploid anddiploid seed. It is also envisioned that good separation of haploid anddiploid seed can be achieved utilizing the high throughput dynamic seedsorting system 10 to obtain only the oil mass data without the seed massdata. Hence, in such embodiments, the microwave resonance cavity 26would not be included in the high throughput dynamic seed sorting system10.

Experiment No. 3

Experimental results of the high throughput dynamic seed sorting system10 operating under typical production conditions with representativematerial are now discussed. Sixty distinct seed populations consistingof a mixture of haploid and diploid seeds 48 were sorted utilizing thesystem 10 by first running a training set subsample for each population.A histogram of oil content for each training set was generated and curvefit using a least-squares fit of the Normal (i.e., Gaussian)Distribution to identify the haploid and diploid components of thepopulation. A typical result of the bimodal oil percentage distributionis shown in FIG. 22 with two Gaussian fits from the least-squaresroutine overlaid and labeled “P1” and “P2”. Peaks “P1” and “P2” in FIG.22 are assigned as putative haploids and putative diploids,respectively.

Haploid recovery (i.e., haploids selected relative to the totalestimated haploids present) and haploid purity (i.e., ratio of haploidseeds to the total seed 48 count in the low oil container) can beestimated from the results of Gaussian curve fitting and the result forthe typical peak fitting shown in FIG. 22 is shown in FIG. 23. Haploidrecovery for a given oil percentage sorting threshold is calculated bynumerical integration of “P1” in FIG. 22 through the sorting thresholdnormalized to the total integrated area of “P1”. Haploid purity for agiven oil percentage sorting threshold is calculated by numericalintegration of “P1” in FIG. 22 normalized to the numerical integrationof the sum of peaks “P1” and “P2” of FIG. 22. In various embodiments,the calculated haploid recovery and haploid purity can be corrected forestimated or measured machine sorting errors whereby seeds 48 selectedfor expulsion by the stray seed removal assembly 130 are unintentionallydiscarded and seeds 48 not selected for expulsion by the stray seedremoval assembly 130 inadvertently bounce into the stray seed catchfunnel 142.

For the present experiment, oil sorting thresholds were selected amongthe complete set of populations to represent a range of predictedhaploid recovery and predicted haploid purity. Complete samples orsubsamples of both sorted categories were planted in the field for eachpopulation in designated plots. After six weeks of growth, emergedindividual plants were phenotypically scored for ploidy by observing theheight of the plant relative to the other plants in the plot. Diploidplants show pronounced differences in height, leaf area, and vigorrelative to their haploid counterparts, and diploid contamination in theputative haploid plot can be estimated by accounting (i.e., eliminating,removing or reclassifying) for plants which were classified as seeds tobe haploid, but were later determined to be diploid plants based ontheir phenotype at approximately six weeks of growth. Similarly, bycounting the smaller and less vigorous members of the putative diploidplot, the rate of seeds mistakenly classified as haploid can beestimated. Using this scoring method, haploid recovery can be estimatedby calculating the fraction of recovered haploids to the total, withappropriate weighting (if subsampling was used). Haploid purity can beestimated by calculating the ratio between the final number of haploidsdetected to the total number of seeds in the putative haploid plot. Bothresults are then compared with the predicted values to demonstrate theability to accurately predict haploid recovery and purity rates using anoil sorting threshold selected from the curve fitting results for atraining set for an arbitrary population. FIGS. 24 and 25 show theobserved vs. predicted haploid recovery and haploid purity,respectively, for the 60 test populations.

The description herein is merely exemplary in nature and, thus,variations that do not depart from the gist of that which is describedare intended to be within the scope of the teachings. Such variationsare not to be regarded as a departure from the spirit and scope of theteachings.

What is claimed is:
 1. A high throughput dynamic seed sorting system, said system comprising: a conveyor assembly including a seed conveyor belt having a plurality of seed cups attached thereto, the conveyor assembly structured and operable to continuously move the conveyor belt at a selected constant rate of speed during operation of the system; a seed feeder assembly structured and operable to singulate seeds from a plurality of seeds and deposit each singulated seed into a respective one of the seed cups as the conveyor belt continuously moves at the selected constant rate of speed; a nuclear magnetic resonance (NMR) assembly having the conveyor belt operably extending therethrough, the NMR assembly structured and operable to generate oil mass data for each seed as each seed moves through the NMR assembly at the selected constant rate of speed; and a computer based central control system structured and operable to: receive the oil mass data from the NMR assembly for each seed, and execute oil content software to: store the oil mass data for each seed and associate the oil mass data received for each seed with the respective seed, and based on the oil mass, compute an oil content value for each respective seed within a time period dictated by the selected constant rate of speed of the conveyor belt.
 2. The system of claim 1 further comprising a microwave resonance cavity structured and operable to receive and have pass therethrough, without pause, each seed after each respective seed has been conveyed through the NMR assembly and to obtain total seed mass data for each respective seed.
 3. The system of claim 2, further comprising a diverter assembly structured and operable to receive the seeds from the microwave resonance cavity and, via commands from the central control system, separate the seeds based on the computed oil/moisture content of each respective seed.
 4. The system of claim 2, wherein the NMR assembly comprises: an NMR rexlameter structured and operable to exert a magnetic force on each seed passing through the NMR assembly; and a radio frequency (RF) probe structured and operable to generate a plurality of pulses and receive an echo from each pulse from which the at least one of oil and moisture mass data is generated.
 5. The system of claim 4, wherein the number of pulses generated each millisecond is such that noise generated by each pulse cancels the noise of subsequent pulses, thereby increasing the signal-to-noise ratio of NMR measurement during determination of the at least one of oil and moisture mass data.
 6. The system of claim 5, wherein the number of pulses generated each millisecond is approximately five pulses per millisecond.
 7. The system of claim 1, wherein each seed cup comprises a reservoir into which each respective seed is deposited by the seed feeder assembly and a plurality of serrations through a body of seed cup, the reservoir and serrations structured and operable to center and reduce vibration of each seed within the respective seed cup and retain each seed in a stable orientation within the respective seed cup as each seed is conveyed through the NMR assembly.
 8. The system of claim 1, wherein the selected rate of speed is approximately one meter per second.
 9. The system of claim 1 further comprising a stray seed removal assembly structured and operable to remove stray seeds that are not retained within a seed cup from the conveyor belt.
 10. A high throughput seed sorting system, said system comprising: a conveyor assembly including a conveyor belt having a plurality of seed cups attached thereto, the conveyor assembly structured and operable to continuously move the conveyor belt at a selected constant rate of speed during operation of the system; a seed feeder assembly structured and operable to singulate seeds from a plurality of seeds and deposit each singulated seed into a respective one of the seed cups as the conveyor belt continuously moves at the selected constant rate of speed; a nuclear magnetic resonance (NMR) assembly having the conveyor belt operably extending therethrough, the NMR assembly structured and operable to generate at least one of oil and moisture mass data for each seed as each seed moves through the NMR assembly at the selected constant rate of speed; a computer based central control system structured and operable to: receive the at least one of oil and moisture mass data from the NMR assembly for each seed, and execute oil/moisture content software to store the at least one of oil and moisture mass data for each seed and associate the at least one of oil and moisture mass data received for each seed with the respective seed; and a diverter assembly structured and operable to receive the seeds and, via commands from the central control system, separate the seeds based on the at least one of oil and moisture mass data of each respective seed.
 11. A method for high throughput seed sorting, said method comprising: continuously moving a conveyor belt at a selected constant rate of speed, the conveyor belt included in a conveyor assembly of a high throughput seed sorting system and having a plurality of seed cups attached thereto; singulating seeds from a plurality of seeds and depositing each singulated seed into a respective one of the seed cups as the conveyor belt continuously moves at the selected constant rate of speed; generating at least one of oil and moisture mass data for each seed as each seed moves through a nuclear magnetic resonance (NMR) assembly at the selected constant rate of speed; and separating the seeds into at least two subsets based on the at least one of oil and moisture mass data of each respective seed. 